Находка теплолюбивого гриба Laetiporus sulphureus на территории г. Кирова

Abstract

The first discovery of edible medicinal mushroom sulfur-yellow tinder on the territory of Kirov was registered. The local climate is characterized by the predominance of continental air of temperate latitudes and the weather is unstable in terms of temperatures and precipitation throughout the year. The region is characterized by a long moderately cold and snowy winter, a prolonged spring, early autumn and a moderately warm short summer. The average air temperature in the city of Kirov in winter is -9.2 оС, in summer it is +16.4 оС. The annual precipitation is 582 mm, of which 60–70% falls on the warm season. A pure culture of sulfur-yellow tinder was isolated using a fragment of the fruit body extracted from the inner part of the basidiocarp. The growth rate of isolate Laetiporus sulphureus NV on wort agar was 10.6 mm/day, on PGA – 7.8 mm/day. Species identification of the fungus was confirmed by molecular genetic method, the results were deposited in GenBank: ON130354.1. Phylogenetic analysis of species of the genus Laetiporus was carried out. The antibiotic activity of the isolate was studied. It was found that the fungus inhibits the growth of gram-positive bacteria i>Arthrobacter humicola 30H, Pedobacter agri 85Td and gram-negative species Pseudomonas cepacia 5809, Erwinia rhapontici DAG1-1. As a result of the liquid-phase ultivation of the L. sulphureus NV isolate on various liquid nutrient media using a magnetic stirrer, it was found that the greatest accumulation of biomass on 20 days of cultivation is observed on a liquid medium based on a decoction of barley husk with the addition of 2% glucose. The accumulation of dry mycelium biomass up to 1.64 g/L was revealed.