초산용액으로 증자한 흑삼의 ginsenoside Rg3 발현 최적화

Abstract

This study aimed to obtain high quality black ginseng with a high content of the ginsenoside Rg3 and melanoidins and low benzopyrene content via nine steaming-drying cycles. The steaming-drying of ginseng was carried out at low pH, and the heat treatment conditions were set to gradually decrease. The steaming-drying conditions were evaluated using response surface methodology. The coefficients of determination (R2) of the models were 0.8926–0.9744 (p<0.05) for the dependent parameters. The response surface for the intensity of the brown color of the black ginseng showed a maximum point at which the brown color intensity was 3.39 (OD at 420 nm) at a mean steaming time of 155.25 min and a mean drying temperature of 57.56°C. The response surface for the ginsenoside Rg3 content of black ginseng showed a saddle point with a maximum ginsenoside Rg3 content of 6.26 mg/g at a mean steaming time of 177.72 min and a mean drying temperature of 65.46°C. However, the response surface for the benzopyrene content of black ginseng showed a maximum point, with the lowest benzopyrene content of 0.22 μg/kg occurring at a mean steaming time of 60.97 min and a mean drying temperature of 56.42°C. The optimal conditions were determined to be a mean steaming time of 175 min and at a mean drying temperature of 68°C; black ginseng manufactured using these conditions yielded a brown color intensity of 2.72 and a Rg3 content of 6.20 mg/g, with a low benzopyrene content of 0.29 μg/kg.