Abstract
Cultivation of rare flora species in order to preserve their gene pool is one of the main tasks of our time. In vitro methods, in combination with ex situ, are nowadays becoming increasingly important means of supporting and maintaining the level of phytodiversity stability. Eucommia ulmoides Oliver is a deciduous tree, belongs to the Eucommiaccac family. This tree species grows in the territory of the NULES of Ukraine Arboretum in a single copy, is a valuable decorative introducer and has a number of medicinal properties. It is currently in poor condition and needs treatment and reproduction for preservation. The purpose of the study was to develop biotechnology of microclonal propagation of E. ulmoides Oliv plants in the initial stages of reproduction for using healthy plant-materials in landscaping and cultivation in botanical gardens. The relevance of microclonal reproduction of E. ulmoides Oliv is substantiated. Features of introduc tion into culture of E. ulmoides Oliv were established with using various sterilizing agents, types of explants and growing conditions. Different types of shoots 15–25 cm long with apical and lateral buds, selected from the donor plant in February – March were used for the work. Murasighe & Skoog (MS) and Driver (DKW) nutrient media were used for cultivation of Eucomia, with cytokinins and auxins addition to their composition: 6-BA (6-benzylaminopurine), NAC (α-naphthylacetic acid) and IAA (β-indolyl-3-acetic acid) both individually and in combination. Sucrose (0.3 %), mesoinositol (100 mg / l) and agar (0.7 %) were added to the medium, the pH of the medium was 5.7–5.8. The influence of different sterilization variants on the development of Eucommia ulmoides Oliver microshoots has been studied. As a result of the research the technique of sterilization of E. ulmoides Oliv explants with 78 % efficiency due to surface sterilization of 0.1 % HgCl2 on annual shoots has been worked out. Optimal nutrient media were established at the stage of in vitro culture of E. ulmoides Oliv. In particular, for the microclonal reproduction of E. ulmoides Oliv., the nutrient medium MS 1 supplemented with 0.5 mg·l-1 BA and 0.1 mg·l-1 NAA is recommended in the initial stages.
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