Ультраструктура кардиомиоцитов крыс c экспериментальным инфарктом миокарда после проведения терапевтической гипотермии и введения мезенхимальных стромальных клеток

Nikolay V Repin,Anna V Trofimova,Boris P Sandomirsky,Larisa N Marchenko,Tatuana P Govorukha,Irina V Belochkina,Nikolay A Chizh

doi:10.15407/cryo27.04.334

Abstract

We studied the ultrastructural changes in cardiomyocytes during necrosis development and re-modelling of the rat heart following experimental myocardial infarction (MI) and performing therapeutic hypothermia and administration of allogeneic mesenchymal stromal cells (MSCs). The infarction was provoked via ligation of left coronary artery. Therapeutic hypothermia was performed in cold chamber for 60 min achieving 4°C skin temperature in the collar zone. The suspension of allogeneic cryopreserved MSCs of placenta with 1.2x10° cells/ml concentration was intravenously administered. In the animals with MI treated with MSCs and a combination of MSCs and hypothermia we revealed the normalization of mitochondrial structure, appearance of small dense mitochondria, the presence of a large number of glycogen granules, testifying thereby to a sufficient oxygen supply into cardiomyocytes and normalization of synthetic processes together with improved microcirculation under MSCs factors. The combination of therapeutic hypothermia with MSCs administration at the background of MI largely promoted the activation of compensatory-regenerative processes in cardiomyocytes. Probl Cryobiol Cryomed 2017; 27(4): 334-347

Highlights

We studied the ultrastructural changes in cardiomyocytes during necrosis development and re-modelling of the rat heart following experimental myocardial infarction (MI) and performing therapeutic hypothermia and administration of allogeneic mesenchymal stromal cells (MSCs)
The small exchange capillaries were located in close proximity to cardiomyocytes
In sarcoplasm of cardiomyocytes we have clearly identified the zones reach on mitochondria clusters: near nuclei, between myofibrils and under sarcolemma

Summary

Introduction

We studied the ultrastructural changes in cardiomyocytes during necrosis development and re-modelling of the rat heart following experimental myocardial infarction (MI) and performing therapeutic hypothermia and administration of allogeneic mesenchymal stromal cells (MSCs). The combination of therapeutic hypothermia with MSCs administration at the background of MI largely promoted the activation of compensatory-regenerative processes in cardiomyocytes. В настоящее время с целью защиты клеток сердца и головного мозга от кислородного голодания в условиях острой коронарной и мозговой недостаточности активно используется терапевтическая гипотермия (ТГ), которая внесена ведущими медицинскими организациями мира в протоколы сердечно-легочной реанимации при острых ишемических состояниях [20, 22]. Мезенхимальные стромальные клетки секретируют многочисленные цитокины и ростовые факторы, стимулирующие выживание, ангиогенез, рост и дифференцировку клеток в зоне ИМ, включая резидентные сердечные стволовые клетки [6, 7, 20, 21]

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Conclusion