Коррекция популяций ценобионтов Bifidobacterium spp. и Lactobacillus spp. у мышей с экспериментальным дисбиозом кишечника после терапии иммобилизованными на энтеросорбентах пробиотиками, хранившимися при –80 и –196°С

Igor P Vysekantsev,Valentina F Martsenyuk,Yulia V Voyda,Kharkiv Medical Academy Of Post-Diploma Education, Kharkiv, Ukraine ,Larisa Ye Shatilova,Olga M Babinets,Andrey V Scheglov

doi:10.15407/cryo25.03.267

Abstract

The research revealed the recovery of the Bifidobacterium spp. and Lactobacillus spp. cenobiont populations in mucin of mice with experimental intestinal dysbiosis after treatment with free and immobilized on enterosorbents probiotics, stored for a year at a temperature of –80, –196°C. Experimental intestinal dysbiosis was simulated by Ampicillin and Metronidazole oral administration. Mice with induced dysbiosis were treated with free cells of S. boulardii, B. bifidum, L. bulgaricus probiotics, the mixtures of free cells with enterosorbents as well as the complexes of probiotic cells immobilized on enterosorbents. The enterosorbents based on activated carbon Sorbex and SCMS-1 were used. Immobilized probiotics have been found to provide a more rapid and complete recovery of cenobiont populations. Low-temperature storage for a year (observation period) and temperature storage regimen did not affect the therapeutic properties of free and immobilized cells of probiotics. The mechanisms of action of immobilized probiotics are under discussion. Probl Cryobiol Cryomed 2015; 25(3):267-286

Highlights

The research revealed the recovery of the Bifidobacterium spp. and Lactobacillus spp. cenobiont populations in mucin of mice with experimental intestinal dysbiosis after treatment with free and immobilized on enterosorbents probiotics, stored for a year at a temperature of –80, –196°C
During 17 days the content of Bifidobacterium spp. and Lactobacillus spp. bacteria in parietal mucin of the animals non-treated with probiotics and enterosorbents was not changed
This study demonstrates that the animals with experimental dysbiosis treated with free S.boulardii cells, mixtures of free cells with enterosorbents and the ones immobilized on enterosorbents had an increased amount of bifidobacteria and lactobacilli in mucin

Summary

Introduction

The research revealed the recovery of the Bifidobacterium spp. and Lactobacillus spp. cenobiont populations in mucin of mice with experimental intestinal dysbiosis after treatment with free and immobilized on enterosorbents probiotics, stored for a year at a temperature of –80, –196°C. It has been shown that immobilization on enterosorbents and storage at –80 and –196°C did not alter the initial biological properties of probiotics and, during the correction of the experimental dysbiosis in immune suppressed mice, the products provided faster recovery of microbiota and eradication of intestinal microflora translocated to internal organs if compared with treatment with free cells and the mixtures of free cells and enterosorbents [2, 3]. To the date there are no comprehensive studies of the effect of immobilized on enterosorbents probiotics stored at different temperatures rendered on the recovery of populations of cenobionts and colonic mucosa mucin of the animals with chemotherapeutic dysbiosis without immune suppression

Methods

Results

Conclusion