원지(遠志)물추출물이 Apoptosis에 미치는 효과

Abstract

ABSTRACT Objectives : The purpose of this study was to observe the effects of Polygalae Radix(PR) on 4-HNE-induced apoptosis in PC-12 cell.Methods : A MTT assay was conducted to observe the cytotoxicity of Polygalae Radix on the cell viability and the cytoprotective effect of Polygalae Radix against 4-HNE that causes oxidative stress-induced cytotoxicity, and then a western blot was conducted to observe the expression of TNF-α, caspase-3, Bax and Bcl-2 protein that are important factors involved with apoptosis signaling pathway.Results : The Polygalae Radix water extract 25 ㎍, 50 ㎍, 100 ㎍ and 200 ㎍/mL had no cytotoxicity on the PC-12 cell. The Polygalae Radix water extract 25 ㎍, 50 ㎍ and 100 ㎍/mL had the cytoprotective effect against 4-HNE that causes cytotoxicity on the PC-12 cell. The P olygalae Radix water extract 50 ㎍/mL significantly suppressed the increase in TNF-α protein expression in PC-12 cell. The Polygalae Radix water extract 25 ㎍ and 50 ㎍/mL significantly suppressed the increase in caspase-3 protein expression in PC-12 cell. The Polygalae Radix water extract 25 ㎍, 50 ㎍ and 100 ㎍/mL suppr essed the increase in Bax protein expression in PC-12 cell but had no significance. The Polygalae Radix water extract 25 ㎍ and 100 ㎍/mL significantly prevented the decrease in Bcl-2 protein expressio n in PC-12 cell, Conclusions : These results suggest that the Polygalae Radix water extract is effective in inhibiting apoptosis.Key words : Polygalae Radix, 4-HNE, TNF-α, caspase-3, Bax, Bcl-2