Влияние условий культивирования Acinetobacter calcoaceticus ІМВ B-7241 на антиадгезивные свойства поверхностно-активных веществ

Abstract

To study the effect of growth factors and microelements in composition of ethanol-, n-hexadecane- and glycerol-containing media on antiadhesive properties of A cinetobacter calcoaceticus IMV B-7241 surfactants. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2:1). The number (%) of attached cells (adhesion) was determined as a ratio of the optical density of the suspension obtained from the materials treated with surfactants to the optical density of the control samples (100 %). Dependence of surfactants antiadhesive properties on presence in the medium of A. calcoaceticus IMB B-7241 cultivation of growth factors and certain microelements, as well as the nature of the carbon source was established. Adhesion of bacteria (Escherichia coli IEM- 1, Bacillus subtilis BT-2) and yeast (Candida albicans D-6) on plastic, dutch tile, linoleum, and steel was a minimal (25-35 %) after surface treatment with surfactant (0.005 mg/ml) synthesized on ethanol in the presence of yeast autolysate and microelements. Replacement the yeast autolysate and microelement mixture in the composition of ethanol- and n-hexadecane-containing media by copper sulfate and iron sulfate and in the medium with glycerol by KCl, zinc sulfate and copper sulfate accompanied by decreasing antiadhesive properties of synthesized surfactants. The obtained data indicate that the increasing surfactant synthesis does not always the accompanied by the formation of product with the required biological properties and indicate the need for studies depending on biological properties of surfactants of the cultivation conditions of producer.