Взаимосвязь между уровнем 25(OH)D3 в крови, количеством противомикробных пептидов в ротовой жидкости и интенсивностью кариеса у молодых лиц

Abstract

Th e aim of the research. correlation analysis between the DMFT index, the content of the active form of vitamin D – 25(OH)D3 in blood and the level of protective proteins in mixed saliva in young individuals with dental caries. Material and methods. The content of vitamin D metabolite in serum of young individuals with dental caries was evaluated using the chemiluminescent immunoassay method. The concentrations of the antimicrobial peptide – cathelicidin LL-37, alpha-defensin 1-3, secretory immunoglobulin A, lipopolysaccharide binding protein (LBP) in oral fluid were determined using the ELISA method. During dental examination, the analysis of dental caries intensity was conducted through calculating the DMFT index. Statistical processing of the study results was carried out with the “IBM SPSS Statistics Version 25.0” (International Business Machines Corporation, USA) soft ware package. Results. A noticeable inverse relationship between DMFT values, the level of 25(OH)D3 in serum and the values of LBP and sIgA in saliva has been revealed. The content of cathelicidin LL-37, alpha-defensin 1-3, secretory immunoglobulin A and LBP in the oral fl uid of young individuals with dental caries decreases against the background of a low level of serum 25(OH)D3, while noticeable direct correlations between the active form of vitamin D and the value of cathelicidin LL-37, alpha-defensin 1-3 and LBP have been registered. Conclusion. Th e established close relations between the carious process intensity, the volume of 25(OH)D3 in the organism and the immune parameters of saliva confi rm the pathogenetic signifi cance of shift s in the system of oral cavity protection factors.