Abstract
It has been found possible to determine the kind of alkaloids contained in ergots by the use of paper partition chromatography. Development of the chromatogram with butanol acidified with acetic acid gives ergometrine or elymoclavine, agroclavine and peptide-type alkaloids in different positions which gave characteristic coloration with the Ehrlich reagent. Furthermore, ergometrine and elymoclavine were separatory determined by development with pyridine-butanol solution. By the application of this procedure with total alkaloids extracted from natural ergots, following results were obtained:
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