コハク酸脱水素酵素比活性測定による制癌剤感受性試験（マウス腎被膜下移植法）の基礎的ならびに臨床的検討 宿主免疫反応の影響と臨床相関

Abstract

Subrenal capsule assay (SRCA) is an in vivo chemosensitivity test which has been reported to be rapid and have high evaluability and clinical correspondence. However, chemosensitivity may be affected by infiltration of inflammatory cells derived from host imm une response and by poor tumor growth.We have developed an SRCA which was evaluated by measuring the specific activity of succinate dehydrogenase (SD). This novel method for evaluating SRCA was tentatively named the “SSDI method” because it was evaluated by determining the inhibition rate of specific activity of SD (SSDI) in a test group relative to a control group. In this investigation, fundamental and clinical studies on the SSDI method were carreid out.In SRCA, relative tumor weight (RW) and total activity of SD increased day by day, but the specific activity of SD (SSD) was almost constant.SSDI method was not influenced by the host immune response, compared with TGIR method.Furthermore, SRCA was carried out for 60 cases of malignant tumors in the oral region which were resected at our department. SSDI method showed a high evaluability rate (91.8%). For patients, the overall positive sensitivity rate was 42.2% by SSDI method and 28.6% by TGIR method. For drugs, the overall positive sensitivity rate was 25.4% by SSDI method and 17.3% by TGIR method. The positive sensitivity rates of anticancer agents evaluated by SSDI method were closer to the clinical sensitivity rates for malignant tumors in the oral region than those obtained by TGIR method. The predicitive accuracy rate was 73.9% by SSDI method and 52.2% by TGIR method.Therefore, SSDI method may be a useful assay to correctly evaluate chemosensitivity in SRCA.