Abstract
Haemolymph and whole-body trehalose contents in overwintering larvae of Enosima leucotaeniella were measured by gasliquid chromatography. The volume of haemolymph (Vμl) was determined by the following relationship: V=(T2/T1)×1, 000, where, T1 is the haemolymph trehalose concentration (mg/ml) and T2 is the whole-body trehalose content (mg). This estimate was comparable in accuracy with the 14C-inulin-dilution method. During October to March 1993 and 1994, the trehalose concentration in haemolymph was controlled by ambient temperature. However, the volume of haemolymph barely changed (55% to 65%) during October to February, but decreased markedly in March. Physiological changes such as decreased body glycogen and increased glucose content were also noticed in March. Therefore, the decreased haemolymph volume may be one phenomenon in a series of physiological changes of overwintering larvae in the late post-diapausing stage.
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