Abstract
Dentures may act as a reservoir for bacteria which cause opportunistic infection such as aspiration pneumonia, and denture cleaning could prevent such an infection. Recently, the bacteriocidal effect of electrolyzed water (EW) on bacteria has been reported.In this study, the influence of EWs on the Candida albicans biofilm in vitro was investigated. C. albicans adhered avidly to type IV collagen. The biofilm was established on the cover slip coated with type IV collagen using Luria broth at 37°C for 5 days. The biofilms were treated with several kinds of EWs, and the efficacy of EWs was investigated.C. albicans well adhered to collagen-coated celldesk, produced glycocalyx-like substance and formed a biofilm after five-day incubation. Scanning electron microscopy revealed degradation of biofilm treated with EW. Vital CFU in the biofilm decreased time-dependently and logarithmically. Five treatments for one minute was more effective than one treatment for five minutes. The effect of EW synthesized by a home care device was equal to or higher than that synthesized by a medical device. These results suggested that EW, even if synthesized by the home care device, is a potent denture-cleanser.
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