Abstract
Oxyradicaldependent chemiluminescence from granulocytes was visualized in rat mesenteric microvascular beds treated with platelet-activating factor (PAF), leukotriene B4 (LTB4) and bacterial chemotactic peptide (FMLP) by means of digital imaging photonic intensified microscopy. The topical application of PAF-acether (100 nM) caused remarkable granulocyte adherence on venular endothelial walls and the subsequent activation of a luminol-dependent photonic burst. Chemilumigenic sites clearly corresponded to the spatial distribution of sticking cells in post-capillary venules. LTB4 and FMLP, however, did not induce any significant photonic burst at concentrations which caused adherent changes between endothelial cells and granulocytes.These findings suggest that PAF may play a role in granulocyte-mediated oxidative stress on venular endothelial cells during acute inflammatory processes, and also provide the first evidence of an intravital oxidative burst of granulocytes in microcirculatory disturbances.
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