法科学的試料からのＤＮＡ抽出におけるＱＩＡａｍｐ ＤＮＡ ｍｉｎｉ ｋｉｔの有用性

Abstract

To evaluate the system of the QIAamp DNA mini kit, supplemented by QIAGEN for DNA extraction, we carried out a comparison of amounts of extracted DNA from various forensic samples. Furthermore, bloodstains were made with 2 μl blood on each of six substrata such as blue denim, wool, mixed spinning, rush (tatami), wood (paulownia), wood (half-split chopsticks), and DNA was extracted from all specimens one day after the specimen preparation. The amount of extracted DNA was estimated by amplified sample peak using of the AmpFL STR profiler kit. The pure DNA were extracted from blood, bloodstain and other forensic samples. However, the amount of extracted DNA by the kit was generally less than that of extracted DNA by the Phenol-Chloroform extraction method, especially for a small sample. In order to obtain enough template DNA with the kit, it is necessary to apply the elution buffer several times on the column. The extracted DNA with the QIAamp DNA mini kit from all the substrata and old bone did not inhibit PCR amplification. We conclude that this DNA extraction system is convenient and valuable for forensic practice.