白板症の免疫組織化学的検討 上皮異形成とＬｅ｀ｙ´抗原およびＰ５３蛋白発現との関連

Abstract

The correlation of epithelial dysplasia with the expression of Lewisy (Ley) antigen and P53 protein was evaluated in oral leukoplakia. Changes in the expression of Ley antigen, P53 protein, and proliferating cell nuclear antigen (PCNA) were examined immunohistochemically in 30 cases of tongue leukoplakia with epithelial dysplasia (mild dysplasia: 11 cases, moderate dysplasia: 10 cases, and severe dysplasia: 9 cases). In addition, the expressions of these molecules in normal tongue epithelia and leukoplakia without epithelial dysplasia were also investigated.The results revealed that Ley antigen was distributed throughout the epithelium except for the basal cell layer in normal epithelium, whereas in leukoplakia it was localized only in the superficial epithelium. P53 protein and PCNA appeared chiefly in the basal cell layer in normal epithelium, while they were more widely distributed in leukoplakia. The positive rate of Ley antigen decreased with the grade of epithelial dysplasia. In contrast, both P53 protein and PCNA positivity increased with the grade of epithelial dysplasia. Specifically, significant changes in the positivity of Ley antigen and P53 protein were observed in leukoplakia, when epithelial dysplasia progressed from mild to moderate dysplasia.These results suggest that mutations of the p53 gene and alterations of cell surface carbohydrates may occur in the early stage at which epithelial dysplasia progress from mild to moderate.