Abstract
The ability of osteoblasts to form bone greatly diminishes with aging, which may be due to the rise of oxygen-derived free radical formation. However, changes that occur in the mineral crystallinity of bone nodules formed by osteoblasts under conditions of oxidative stress have not been sufficiently clarified. The aim of this study was to elucidate the effects of oxidative stress on osteogenesis. We investigated bone nodule formation in an osteoblast-like cell line, MC3T3-E1, using von Kossa staining and micro-X-ray diffraction (micro-XRD). For the micro-XRD examinations, the bone nodules were analyzed after removal from the culture plate and placed on a slide glass or a silver membrane filter. We found that nodule formation was lower on day 30 following treatment with H2O2 and mineral crystallinity in H2O2-treated cells was also lower than in the controls. These results suggest that osteoblasts damaged by oxygen free radicals produced during the aging process show a decline in their bone nodule formation ability.
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