Abstract
In this study, we characterized extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae isolated from clinical specimens in Korea and found two strains harboring plasmid-mediated blaSHV-11, Klebsiella pneumoniae. First, the isolates were detected using the Vitek system and confirmed by the double-disk synergy test. The classification of gene coding for ESBL was also performed by polymerase chain reactions and followed by DNA sequencing. The transmission of genes was confirmed by transconjugation and transformation. Resistant expression of transformants was determined by broth microdilution minimal inhibitory concentration test. Genotypic analysis revealed that one strain harbored the bla TEM-1 , bla SHV-11 and blaCTX-M-15 and the other strain harbored the bla SHV-11 and bla CTX-M-15 . They showed high resistance to oxyiminocephalosphorins (3rd-generation cephalosporins), while the transformant containing only bla SHV-11 did not show any resistance to the antibiotics.
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