СИНТЕЗ МЕЛАНІНУ ЧОРНИМИ ДРІЖДЖАМИ PSEDONADSONIELLA BRUNNEA: ЗАЛЕЖНІСТЬ ВІД КІЛЬКОСТІ L-ТИРОЗИНУ В КУЛЬТУРАЛЬНОМУ СЕРЕДОВИЩІ

Abstract

To determine the optimal conditions for the synthesis of melanin by black yeast fungi Pseudonadsoniella brunnea (Basidiomycota, Agaricomycotina, Agaricomycetes, Polyporales, Meripilaceae), depending on the amount of L-tyrosine in the culture medium was the purpose of the work. The standard Malt Extract Broth (MEB) liquid nutrient medium was used within this study. L-tyrosine was added to the culture medium in a quantity of 0.01, 0.025 and 0.05%.To obtain the melanin the cultivation of Pseudonadsoniella brunnea was carried out at pH 1-1.5, temperature + 21 ± 1 ° C during 7 days. Statistical processing of the results was carried out using generally accepted methods of variation statistics. It has been established that the level of melanin synthesis by black yeast-like fungi Pseudonadsoniella brunnea depends on the amount of L-tyrosine introduced into the culture medium. The MEB nutrient medium containing 0.05% L-tyrosine in this series of experiments found to be the best composition for obtaining melanin by the strain-producer Pseudonadsoniella brunnea. Compared to control (MEB without L-tyrosine), the amount of melanin synthesized by Ps. brunnea in these conditions increased by 2.5 times. The further research into the optimal conditions for the cultivation of black yeast-like fungi Pseudonadsoniella brunnea in order to obtain melanin is relevant and promising.