바이오디젤 생산을 위한 규조류 Phaeodactylum tricornutum의 지질 추출 전처리 공정 최적화

Abstract

Phaeodactylum tricornutum is an emerging diatom in the microalgal biotechnology field for biofuel application. P. tricornutum B2089 was incubated for 13 days in a 3 L flask containing f/2 medium mixed with oceanic sediment extracts. To select the most effective pretreatment process (cell destruction method) of P. tricornutum B2089 for lipid extraction, various mechanical methods including autoclave, bead beating, sonication, French-press, and microwave were tested. Of these, enhanced lipid extraction was obtained through microwave treatment (1200W for 20 minutes at 2450 ㎒, 150℃). Under this condition, the lipid content from P. tricornutum B2089 was 53.3% (w/w) of dry cell weight or 3.4 g/L and the lipid contained palmitoleic acid (C16:1) and oleic acid (C18: 1) as major fatty acids.