Abstract
In this study, we did the molecular typing of 39 environmental Legionella pneumophila serogroup 1 isolates collected from 2001-2003 in Busan using the pulsed-filed gel electrophoresis (PFGE). PFGE of SfiI fragments were divided into 10 pulsotypes (A~J), corresponding to ＜65% similarity and a subtype within each pulsotype was characterized by ＞84% similarity. The major cluster was pulsotype E (46.2%), which included 18 isolates and was divided into 4 subtypes (E1~E4). PFGE of NotI fragments were divided into 8 pulsotypes (a~h), corresponding to ＜60% similarity and a subtype within each pulsotype was characterized by 100% similarity. The major cluster was pulsotype f (38.5%), which included 15 isolates. The ATCC type strain L. pneumophila serogroup 1 was identified as a different molecular pulsotype compare to the Busan isolates. It is possible that L. pneumophila serogroup 1 isolated in Busan with specific DNA pattern is comparable with those isolation in other cities in Korea.
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