Поиск полиморфных вариантов гена LCORL с помощью секвенирования по Сенгеру у пород кур различного направления продуктивности

Abstract

Abstract. The effect of polymorphic variants of the LCORL gene in many species of farm animals was revealed. It is believed that the LCORL gene is associated with skeleton sizes in chickens, but has not yet been adequately studied. Therefore, we are faced with the task of finding new polymorphic variants in the LCORL gene in gene pool breeds. The aim of the work is to search for and analyze polymorphic variants in the LCORL gene using Senger sequencing in breeds of various types of productivity. Methodology and methods. The studies were carried out on the basis of the laboratory of molecular genetics of All-Russian Scientific Research Institute of Genetics and Breeding of Agricultural Animals. The object of the experiment was populations of 4 breeds of chickens of different directions in productivity of the All-Russian Scientific Research Institute of Genetics and Breeding of Agricultural Animals Biological Resource Collection “Genetic Collection of Rare and Endangered Breeds of Chickens” (Pushkin, St. Petersburg): Kornish, Kitayskaya shelkovaya, Ital’yanskaya kuropatchataya, Pushkinskaya. 61 DNA samples served as the material for the study. amplification was performed on a Thermal Cycler T100 instrument (Bio-Rad, USA). Nucleotide sequences were determined on an Applied Biosystems 3500 automated sequencer (Thermo Fisher Scientific Inc., USA) at the All-Russian Scientific Research Institute of Genetics and Breeding of Agricultural Animals Laboratory of Molecular Genetics. The Big Dye Terminator Cycle Sequencing Kit (Applied Biosystems, USA) was used for sequencing. Biometric data processing was performed using Microsoft Excel. Results. The study revealed genetic variation in polymorphic variants A30G, G82C, G236T, A342G, A450C, A503G, A508G in the intron of the LCORL gene of chicken populations of different directions of productivity. The results suggest that the polymorphic A30G variant of the LCORL gene can be considered as a DNA marker for the sign of “skeleton size” in chickens.