Abstract
JSAP1 and JSAP2 are structurally close related family proteins and originally have been identified as scaffold proteins of the JNK and p38 MAPK modules. The specificity of MAPK cascades is regulated, at least in part, by scaffold proteins, such as JSAPs[1-7]. Some studies showed [7-12] that JSAPs function as adaptor proteins which link cargoes to kinesin-1, the cellular cargo transporter along with microtubules. Also JSAPs regulate certain crucial neuronal processes, particularly axon elongation, branching and neurite outgrowth[13-22]. Interestingly, in recent studies it was reported that an elevated expression of JSAP2 protein in various cancers, including breast, colorectal cancer and hepatocellular carcinoma, might be proposed as a biomarker for diagnosis of cancers at early stage[25-28]. However, roles of JSAP in cancer cells and their underling molecular mechanisms are not known well at present. Within our project which aims to clarify potential roles of JSAPs in cancers, in the present study an overexpression of JSAPs in HeLa cells using lentiviral vectors were performed and immunocytochemical analyses focused on centrosomes at mitotic stage of dividing cells were done. In results, in HeLa dividing cells, which overexpress the wild type of JSAPs or their substitution mutant forms a multiple centrosome at mitotic stage of cells were found. However, in case of overexpression of the deletion mutant JSAP proteins in cancer cells a multi-centrosome abnormality were not observed, as well as in control cells.
Highlights
Within our project which aims to clarify potential roles of JSAPs in cancers, in the present study an overexpression of JSAPs in HeLa cells using lentiviral vectors were performed and immunocytochemical analyses focused on centrosomes at mitotic stage of dividing cells were done
In HeLa dividing cells, which overexpress the wild type of JSAPs or their substitution mutant forms a multiple centrosome at mitotic stage of cells were found
Одоогоор хорт хавдрын эсэд JSAP уургуудын гүйцэтгэх үүрэг, тэдгээрийн молекул механизм бүрэн тодорхой болоогүй байгаа хэдий ч олон судлаачид JSAP2 уургийг хавдрын оношлогоонд биомаркер болгон хэрэглэхийг санал болгох болов[25,26,27,28]
Summary
P38MAPK-аар сигнал дамжих үед угсрагч скаффолдын үүрэг гүйцэтгэдэг. Харин JSAP1 нь JNK-д, JSAP2 нь p38-д илүү өвөрмөцөөр харилцан үйлчилдэг байна[7,8,9,10,11,12]. Мөн JSAP уургууд нь эсийн дотор карго зөөвөрлөдөг мотор уураг болох кинезин-1 -тэй (kinesin-1) харилцан үйлчилж улмаар каргог мотор уурагтай холбодог болохыг судлаачид тогтоожээ [1316]. Түүнчлэн эсийн митоз хуваагдлын прометафаз үе болон цитокинезийн үед JSAP уургууд чухал үүрэгтэй болох нь тогтоогдоод байна[23,24]. Хавдрын молекул биологийн сүүлийн үеийн зарим судалгаанууд[25,26,27,28] хөхний, бүдүүн гэдэсний, элэгний гэх мэт олон төрлийн хавдрын үед JSAP2 уургийн экспресс нэмэгддэг болохыг тогтоосон нь ихээхэн сонирхол татаж байгаа юм. Одоогоор хорт хавдрын эсэд JSAP уургуудын гүйцэтгэх үүрэг, тэдгээрийн молекул механизм бүрэн тодорхой болоогүй байгаа хэдий ч олон судлаачид JSAP2 уургийг хавдрын оношлогоонд биомаркер болгон хэрэглэхийг санал болгох болов[25,26,27,28]. Бидний энэхүү ажлын зорилго нь хавдрын эсийн хуваагдлын үед JSAP уургийн гүйцэтгэх үүргийг судлахад оршсон болно
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