Abstract
S. H. Lee* and I. J. Seo**Department of Animal Science, Korea National Agricultural College, RDA, Suwon 445-893, Korea*Woo Tech Co., Yongin 102-104, Korea**ABSTRACTThis study was conducted to determine effects of supplementation levels of aqueous direct-fed microbials(DFM; Bacillusspp.) to TMR(exp. 1.) and aqueous DFM addition under the various ratios of starch andcellulose(exp. 2.) on ruminal fermentation and fibrolytic enzyme activity. In experiment 1, ruminal fluidstaken from rumen-cannulated Holstein cows were incubated during 24 hr by using TMR as substrates.Aqueous DFM was applied at a rate of 0, 0.025 and 0.05%, respectively. The pH of 0.025% treatmentwas not significantly different from that of control at 6 and 9 hr, but it was significantly lower (P<0.05)than 0.05% treatment.Concentrations of ammonia-N and VFAs were not affected by supplementing aqueous DFM. The A:Pratio of 0.05% treatment was significantly increased(P<0.05) by supplementation of aqueous DFM ascompared with that of control at 24 hr. Although overall fibrolytic enzyme activities were not significantlyaffected by supplementing aqueous DFM, CMCase(carboxymethylcellulase) activity showed significantincrease(P<0.05) compared to control at 6hr. However, the xylanase activity of 0.05% treatmentsignificantly decreased(P<0.05) at 12 hr due to the application of aqueous DFM. There was no significantdifference for invitro dry matter disappearance among treatments.In experiment 2, ruminal fluids were incubated under the condition of various ratios of starch tocellulose (90:10, 70:30, 50:50, 30:70 and 10:90) with or without aqueous DFM(0.025%). Ruminal pH wasCorresponding author:S. H. Lee, Department of Animal Science, Korea National Agricultural College, RDA,Suwon 445-893, Korea. Tel:+82-11-9639-5037, Fax:+82-31-229-5055. E-mail:sh5037@hanmail.net
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