Abstract

The results of studies of the morphogenetic responses of soybean culture in vitro , differing in photoperiodic sensitivity are presented. Research has been carried out on 7 isogenic genotypes for the genes E-series ( early maturity genes ) lines (NILs) of soybean Glycine max (L.) Merrill varieties Сlark. It has been shown that all the lines have been efficiently in culture in vitro , able to direct morphogenesis using cotyledon explants as nodes, but differ in the degree of manifestation and direction of morphogenetic responses. Isolines with shortday photoperiodic reaction (SDP) implement morphogenetic reaction in vitro by gemogenesis and rhizogenesis ways, while the photoperiodic neutral isolines (FNP) – increasingly realized by callusogenesis. It has been found that the SDP isogenic lines have been characterized by the highest rates of adventitious shoot formation, root formation and the formation of a larger number of shoots on explant on various regeneration media than photoperiodic neutral isogenic lines. The relationship between the photoperiodic response isogenic lines in vivo , which is determined by genotype, and features of morphogenesis in vitro are discussed.

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