수온 스트레스에 의한 명태의 Heat Shock Factor 1의 분자생물학적 특성과 Heat Shock Protein 70의 단백질 발현 패턴

Abstract

This study evaluated the levels of mRNA expression of HSF1 and HSP70 expression patterns in walleye pollock, Gasus chalcogrammus according to water temperature. Walleye pollack HSF1, which has not been reported, was used as a Rapid Amplification of cDNA Ends (RACE)-PCR strategy. The HSF1 amino acid sequence of walleye pollack showed 93% identification with the Atlantic cod. On the other hand, it showed relatively low identification with 50% in zebrafish. However, in the phylogenetic analysis, fish HSF1 formed a single clade group. The relative mRNA expression of HSF1 in the liver, intestine, gill, kidney, and heart of juveniles and immatures was investigated. The tissues in juvenile and immature, HSF1 mRNA expression was the highest at 14℃, and HSF1 mRNA levels increased significantly from 11℃ compared to 5 and 8℃. The mRNA levels of HSF1 were particularly high in the gill both juveniles and immatures. The expression of HSP70 was also evident in 11℃ and 14℃. These results indicate that water temperature of 11°C or higher is exposed to stressed-condition in walleye pollock. Therefore, HSF1 and HSP70 can be considered as important indicator for water temperature stress in walleye pollock.