ПОИСК ИСТОЧНИКОВ УСТОЙЧИВОСТИ К GLOBODERA PALLIDA И К PVX В КОЛЛЕКЦИИ ОТЕЧЕСТВЕННЫХ СОРТОВ КАРТОФЕЛЯ С ИСПОЛЬЗОВАНИЕМ МОЛЕКУЛЯРНЫХ МАРКЕРОВ

Abstract

Great damage to potato production is caused by cyst nematodes, which include two species: Globodera pallida (Stone) Behrens, the object of external quarantine, and Globodera rostochiensis (Wollenweber) Behrens, the object of internal quarantine in the Russian Federation. The breeding of varieties resistant to the G. rostochiensis has long been one of the priorities of Russian potato breeding, while a targeted search for sources of resistance to G. pallida in our country until recently was not actually carried out, since this pathogen was not detected in Russia, although it was traced in neighboring countries. The possibilities of selection of G. pallida -resistant genotypes using traditional phytopathological methods are extremely limited, so the information about the presence of markers of resistance genes to this pathogen in the domestic breeding gene pool is of particular importance. Here we present the results of molecular screening of 160 varieties bred in Russia and in adjacent countries from the collection of VIR for the presence of markers of Gpa2 gene, which provides resistance of potato varieties to G. pallida (Pa2/Pa3 pathotypes). It is shown that among 160 varieties of the analyzed subset 19 have a di­agnostic fragment of the allele-specific marker of the Gpa2 gene - Gpa2-2. These 19 varieties isolated in molecular screening simultaneously con­tained allele-specific markers of the Rxl gene controlling resistance to potato virus X (PVX).