Abstract
기능성 한천올리고당 생산에 사용할 수 있는 유전자원을 확보하기 위하여 동해안 기장 해수에서 한천분해활성을 보이는 해양유래 세균 SL-12를 분리하였으며 165 rDNA염기서열 분석으로 해양기원의 Glaciecola 속과 가장 유사한 균주임을 확인하였다. SL-12 균주가 생성하는 한천분해효소(agarase)의 최적 pH는 pH 7.0 (20 mM sodium phosphate 완충용액)이고 최적 온도는 <TEX>$30^{\circ}C$</TEX>로 나타났다. 분리 된 Glaciecola sp. SL-12 균주가 생산하는 한천분해효소의 분해산물에 대한 TLC 분석결과, 기능성 한천올리고당을 생산하는 <TEX>${\beta}$</TEX>-agarase로 판명되어 산업적 활용 가능성이 높은 것으로 기대된다. A novel agar-degrading bacterium SL-12 was isolated from seashore of Kijang at Busan, Korea, and cultured in marine broth 2216 media. Isolated bacterium SL-12 was identified as Glaciecola genus by 16S rDNA sequencing with 98% identity. The optimum pH of the enzyme activity was 7.0 and the optimum temperature for the reaction was <TEX>$30^{\circ}C$</TEX>. The enzyme hydrolyzed neoagarohexaose to yield neoagarobiose as the main product, indicating that the enzyme is <TEX>${\beta}$</TEX>-agarase. Thus, isolated bacterium and the enzyme would be useful for the industrial production of neoagarobiose.
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