마이크로웨이브로 추출한 용매별 치자(Gardeniae Fructus) 추출물의 생리활성

Abstract

Dept. of Food Science and Technology, Kyungpook National University, Daegu 702-701, KoreaAbstractGardeniae Fructus (GF) is a fruit of Gardenia jasminoides Ellis (Rubiaceae) which has been used in traditional medicine. It contains not only geniposide but also resveratrol, a kind of stilbene as a natural antioxidant. In this study, we investigated physiological activities of GF extracts by measuring electron donating ability (EDA), nitrite scavenging ability (NSA), super oxide dismutase (SOD)-like activity and inhibitory effect of tyrosinase activity (ITA). As a solvent, it was extracted with water, 50 and 100% ethanol using microwave. At the concentration of 6 mg/mL, EDA was the highest at 50% ethanol extract but the lowest at 100% ethanol ±extract, 96.43±0.25% and 77.06 0.22% respectively. NSA showed the strongest activity at 50% ethanol ±extract like EDA (6 mg/mL: 75.26 0.28%). ITAs were below 20% in all samples and their activities reduced according to increasing concentration. Likewise, SOD-like activities decreased with increasing concentration of extracts in all samples. In conclusion, GF extracts showed low SOD-like activities and ITAs but EDAs and NSAs were fairly high; especially EDA of 50% ethanol extract (6 mg/mL) was high as those of 0.1 and 1% L-ascorbic acid. Therefore the results suggest that GF extracts may be useful as potential sources of natural antioxidants.Key words: Gardeniae Fructus, physiological activity, microwave-assisted extraction