Abstract
The influence of He-Ne laser radiation (632.8), incoherent monochromatic red light (601-685 nm), as well as pulsed radiation semiconductor laser (660 nm) on the growth of the culture of wild type or uvrA strain of E. coli WP2 and on the DNA transformation of plasmid pDick (Amp, Km) in E. coli NM522 was investigated. After light signal, photoenhancement of the quantity of cells in culture retains during several generations, while the enhancement of the transformation efficiency was observed only for the kanamycin marker. This makes it possible to suppose that metabolic signaling network acts as a red light receptor. The observed spectral selectivity suggests that flavohemoglobin fatty acid peroxidase works as a photosensitive component in the reparative network. It is proposed that the enhanced effectiveness of light in the uvrA strain results from photoactivation ofglobin EcDOS phosphodiesterase as an O2 provider for AlkB demethylase.
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