치자(梔子)가 대식세포의 Cytokine에 미치는 영향

Abstract

Objectives: The purpose of this study was to investigate the effects of Gardeniae Fructus Water Extract (GF) on the production of inflammatory mediators in RAW 264.7 cell treated with lipopolysaccharide (LPS). Methods: Gradeniae Fructus was extracted with distilled water (2,000 ml) for 2 hours. In order to evaluate cytotoxicity of GF, 3 - (4,5-dimethylthiazol-2-yl) - 2,5 - diphenyltetrazolium bromide (MTT) assay was performed. To investigate antiinflammatory effects, the concentration of nitric oxide (NO) was measured with No assay, calcium (Ca) was measured with Fluo-4 Ca assay, and cytokine was measured by Bio-Plex cytokine assay in RAW 264.7 cell. And when p-value is below 0.05, it is judged to have the significant difference statistically. Results: 1. GF did not show any cytotoxicity. 2. GF suppressed the production of NO and Ca at the concentration of 25, 50, 100 and <TEX>$200{\mu}g/ml$</TEX>. 3. GF suppressed the production of interleukin (IL)-<TEX>$1{\beta}$</TEX>, IL-10, IL-12p40, macrophage-colony stimulating factor (M-CSF), macrophage inflammatory protein (MIP)-<TEX>$1{\beta}$</TEX> and keratinocyte chemoattractant(KC) at the concentration of 25, 50, 100 and <TEX>$200{\mu}g/ml$</TEX>. 4. GF suppressed the production of vascular endothelial growth factor (VEGF), granulocyte-colony stimulating factor (G-CSF) and monocyte cheomattractant protein (MCP)-1 at the concentration of 25, 50 and <TEX>$100{\mu}g/ml$</TEX>. 5. GF suppressed the production of granulocyte macrophage-colony stimulating factor (GM-CSF) and regulated on activation, normal T cell expressed and secreted (RANTES) at the concentration of 25 and <TEX>$50{\mu}g/ml$</TEX>. 6. GF suppressed the production of MIP-2 at the concentration of 50 and <TEX>$100{\mu}g/ml$</TEX>, and tumor necrosis factor (TNF)-<TEX>${\alpha}$</TEX> at the concentration of 50 and <TEX>$200{\mu}g/ml$</TEX>. Conclusions: These results suggest that GF has anti-inflammatory effect and immuno-modulating activity.