МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКАЯ ХАРАКТЕРИСТИКА ШТАММА CUTIBACTERIUM (PROPIONIBACTERIUM) ACNES A1-14 КАК ПРЕДСТАВИТЕЛЯ СИМБИОТИЧЕСКОЙ МИКРОБИОТЫ ЧЕЛОВЕКА

Abstract

The bacteria of the Cutibacterium (formerly Propionibacterium) acnes species are representatives of normal microbiota of human skin on the one hand and they contribute to the development of inflammatory processes, such as acne, infectious complications in the post-traumatic and postoperative period of the eye (endophthalmitis), the cardiovascular system (endocarditis), central nervous system and musculoskeletal system (osteomyelitis), implant-associated infections on the other hand. To identify epidemically significant genotypes with high pathogenetic potential and genotypes of low pathogenic commensals the different molecular genetic approaches are used. The whole genome sequencing of C. acnes A1-14 strain isolated from the large intestine of a healthy person was carried out for the first time. As a result of alignment and collection, the nucleotide sequence of the genome was 2,484,560 pairs of nucleotides.The strain belongs to a phylotype II, a ribotype 6 and sequence-type 7 (McDowell A. et al. scheme) or 73 (Lomholt H. B., Kilian M. scheme) according to the results of genotyping. We showed that there are no mobile elements, determinants of pathogenicity and antibiotic resistance, mutations in the 16S rRNA, 23S rRNA, gyrA, gyrB, parC, parE genes and established the presence of CRISPR-Cas structure in the genome of C. acnes A1-14. An extrachromosomal sequence belonging to the genome of the Syphoviridae family bacteriophage was found. The morphological characteristic of the bacteriophage virion, the presence of that as extrachromosomal structure is typical to many C. acnes strains, is given using the electronic microscopic method of research. Thus, the obtained in our study results complement the understanding of the molecular genetic features of C. acnes strains isolated from various human biotopes and which are symbiotic microbiota representatives.