Abstract
Department of Herbal Crop Research, NIHHS, RDA, Eumsung 369-873, Korea.ABSTRACT : This study was carried out to evaluate the preventive effect of three forms of Korean ginseng roots (fresh,white and red) against bisphenol A (BPA) toxicity in mouse male germ cells (GC-2spd, TM3, TM4). ROS (reactive oxygenspecies) generation were measured by DCF-DA (2’,7’-dichlorohydrofluorescein diacetate) assay. Also, semi-quantitativereverse transcriptase polymerase chain reaction (RT-PCR) was performed to quantify the mRNA expression levels of apop-tosis-related genes, Bax (pro-apoptotic gene) and Bcl2 (anti-apoptotic gene). ROS generation was increased by 50µM BPA,but definitely decreased by treatment with Korean ginseng extracts (fresh, white and red) in mouse male germ cells. In espe-cial, Korean fresh ginseng extract reduced significantly ROS production to normal control. In addition, Korean fresh andwhite ginseng extracts suppressed the apoptosis of mouse male germ cells by fine-tuning mRNA levels of apoptotic geneschanged by BPA. In general, Korean fresh ginseng extract was more effective than white ginseng extract for reducing BPA-induced oxidative stress and apoptosis in mouse male germ cells. Therefore, Korean fresh and white ginseng may help toalleviate biphenol A toxicity in mouse male germ cells.Key Words : Panax ginseng, Bisphenol A, Germ Cell, Apoptosis, Reproductive Toxicity
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
