Abstract
Dept. of Food Science and Technology, Chungbuk National University, Chungbuk 361-763, KoreaAbstractThe objective of this study was to evaluate the effect of defatted grape seed extract (DGSE) on adipocyte differentiation in 3T3-L1 preadipocytes. DGSE at 100 μg/mL significantly suppressed lipid accumulation and glycerol-3-phosphate dehydrogenase activity in hormonally stimulated adipocytes, an indicator of adipocyte differentiation. In order to understand the anti-adipogenic effects of DGSE, the changes in the expression of several adipogenic transcription factors including peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/ enhancer-binding protein (C/EBP) α and β were investigated using immunoblotting. DGSE suppressed the expression of PPARγ, C/EBPα, and C/EBPβ proteins compared with control adipocytes in a dose-dependent manner. This results indicated that DGSE may alter fat mass by directly affecting adipogensis in maturing preadipocytes and thus may have applications for the treatment of obesity.Key words: defatted grape seed extract, 3T3-L1, adipogenesis, PPARγ, C/EBP
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