ミヤママタタビ（Ａｃｔｉｎｉｄｉａ ｋｏｌｏｍｉｋｔａ）培養体由来根組織からの器官形成，不定はい誘導ならびに植物体再生

Abstract

Root segments derived from cultures of Actinidia kolomikta were cultured in vitro on BW medium (Sugawara et al., 1994). Twelve combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (0, 0.1, 1 and 10μM) and zeatin (0, 1 and 10μM) were tested at 25°C, under a 16-hr photoperiod with a light intensity of 70μmol·m-2·s-1. Calli formed from root segments at all concentrations of 2, 4-D and zeatin. Of the adventitious buds which formed from the callus, 92% developed into shoots with a combination of 0.1μM 2, 4-D and 10μM zeatin. Root segments excised from cultures formed the most somatic embryos when cultured with 10μM of 2, 4-D and zeatin. When young leaflets with roots were cultured on the BW medium with zeatin, adventitious buds formed on the root tip. The rate of adventitious bud formation increased with an increase in zeatin concentration. The adventitious buds developed into shoots ; shoots rooted and grew into vigorous plantlets when transferred onto Miller's medium with 1μM 1-naphthaleneacetic acid (NAA).