백굴채 추출물의 항산화 활성과 기전

Abstract

*Department of Biochemistry, Hallym University Medical School, Chuncheon 200-702, Korea.**Institute of Natural Medicine, Hallym University Medical School, Chuncheon 200-702, Korea.***Department of Food and Nutrition, College of Natural Sciences, Hallym University, Chuncheon 200-702, Korea.****Institute of Cell Differentiation and Aging, Hallym University Medical School, Chuncheon 200-702, Korea.ABSTRACT : Chelidonium majus (CM) contains several isoquinoline alkaloids that have been reported to have various bio-logical activities such as anti-inflammatory, antimicrobial, antioxidant, immune-modulatory, and antitumoral. It has beenreported that the extract of CM had an antioxidant potential, however the mechanism has not been verified. In this study,we found that CM extract activated FOXO3a. FOXO3a is a transcription factor that involved in various biological processessuch as cell cycle arrest, apoptosis, DNA repair, and ROS detoxification. Transcriptional activities of FOXO3a were regu-lated by post-translational modifications including phosphorylation, acetylation, and ubiquitination. Protein level ofFOXO3a was increased by CM extract. Promoter activities of FOXO-transcriptional target genes such as MnSOD, p27 andGADD45 were activated by CM extract in a dose dependent manner. In addition, protein level of MnSOD, major antioxi-dant enzyme, was increased by CM extract. Thereby ROS level was decreased by CM in old HEF cells. These results suggestthat CM extract has an antioxidant activity through FOXO activation. Key Words : Chelidonium majus, FOXO3a, Antioxidant