Иммуногистохимическое исследование иннервации надпочечника крысы

Abstract

The aim of the study was to study the innervation of the cortical part and medulla of the rat adrenal gland using neuroimmunohistochemical markers. Original data on the innervation of the rat Wistar (n = 8) adrenal cortex and medulla were obtained using immunohistochemical detection of the PGP 9.5 protein, synaptophysin, tyrosine hydroxylase, peripherin and serotonin. It was established that all zones of the cortex (glomerular, bundle, and reticular) are innervated by nerve fibers of various neurotransmitter identity. In the cortex, the innervation of the glomerular zone is most pronounced. A thick terminal plexus of varicose axons around islets of endocrinocytes, arterioles and nerve cells was detected in this zone. Cholinergic nerve structures (plexus of preterminal nerve fibers and the terminal synaptic network, which consists of varicose parasympathetic axons located around groups of neuroendocrine cells and sinusoidal capillaries) prevail in the medulla. Parasympathetic and sympathetic intramural ganglia were found in the medulla. Chromaffin cells immunoreactive to various catecholamines and serotonin were found in the adrenal medulla. It was assumed that different sources of sympathetic innervation of the organ are present. These are the medulla's own neurons and sympathetic trunk neurons. Such innervation is important for the regulation of local blood circulation.The results obtained in this study can be used both in scientific research and in the diagnosis of adrenal diseases.