Abstract

본 연구는 감마방사선 조사에 의해 기능성 물질이 증가하고, 생리적 특성이 증진된 느티만가닥 버섯의 새로운 품종을 개발하기위하여 수행되었다. 돌연변이 유기를 위하여 느티만가닥 버섯의 갈색 계통 균주 HYM-056의 원형질에 감마방사선을 조사하여 2,000개의 돌연변이체를 무작위로 선발하고 병 재배하여 자실체를 형성시켰다. 이 중 생장속도가 빠르고, 중량이 무거우며, 자실체를 다량으로 생산하는 500개 균을 선발하였다. 선발된 균주의 자실체 형성을 위하여 미강, 보리껍질, 미송이 함유된 플라스틱 병에 재배하였다. 접종 100일 후, 자실체의 특성을 조사하였다. 그 결과 갓의 색깔, 형태, 크기와 대의 길이, 직경, 숫자, 무게 등에 따라 6개의 그룹으로 나뉘었다. 또한 URP-PCR 핵산 지문 분석으로 유전적 변이를 조사하였다. This study was performed to develop new Hypsizygus marmoreus cultivars that have enhanced functional materials and improved physiological characteristics with mutagenesis by gamma ray irradiation. Protoplasts of H. marmoreus brown strain HYM-056 were irradiated by gamma ray for mutagenesis, and then 2,000 clones of mutants were randomly selected and the fruiting bodies were induced by bottle culture. Among them, 157 isolates with fast-growing, heavy and many fruiting body-producing were selected. The isolates were cultured in plastic bottle containing rice bran, barley hulls and fir sawdust to form the fruiting bodies. About 100 days after inoculation, characteristic of fruiting bodies were investigated. The isolates were divided into 6 groups based on color, shape and size of pileus, and length, diameter, number and weight of stipe. In addition, the genetic variation of the isolates was analyzed by URP-PCR fingerprinting.

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