ГРАМИН И ЕГО ТОКСИКОЛОГИЧЕСКАЯ ОЦЕНКА

Abstract

The aim of the research was to study the acute and subchronic toxicity of the drug gramine in white mice. The micromycete Drechslera graminea served as the basis for the creation of the gra-mine preparation. Experiments to determine the acute toxicity of the preparation gramine were car-ried out on outbred white mice (males) weighing 18-20 grams. The drug was administered to ani-mals intraperitoneally, once in doses from 400 to 2400 mg/kg. 6 mice were used for each dose. The condition of the animals was monitored for 14 days. The dose causing the death of 50 % of the an-imals (LD50) and the absolutely lethal dose (LD100) were determined. LD50 was calculated by the Kerber method. The study of subchronic toxicity of gramine was carried out for 30 days. The drug was administered to 10 mice intramuscularly daily, in the first 4 days, gramine was injected at a dose of 0.1 LD50, and then every 4 days the dose was increased 1.5 times and finally it was 1520 mg/kg. To determine the cumulative properties of the drug in white mice, a "subchronic toxicity" test was used. The reliability of the results obtained was taken into account in accordance with the Student's t-criterion at P <0.05. As a result, it was found that the average lethal dose (LD 50) of gramine for white mice is 1334 mg/kg, LD 100 - 2400 mg/kg. Studies of the subchronic toxicity of gramine showed that doses of the drug from 133 to 450 mg/kg did not cause significant changes in the behavioral responses of laboratory mice, and a pronounced toxigenic effect began to appear at a dose of 675 mg/kg and continued to increase after a further increase in doses of gramine (1013-1520 mg/kg), while causing mortality reaching the level of 50 %. It was found that gramine with intraperi-toneal administration of the LD50 of gramine for white mice corresponds to 1334 mg/kg, which al-lows the drug to be classified as a low-toxic substance. The cumulation coefficient of gramine is 1.14, which corresponds to the group of drugs with pronounced cumulation.