Abstract
The cells of Streptoinyces roseochromogenes NRRL-B-1233 were immobilized in photo-crosslinked resin, 2, 2-bis[4-[methacroyloxypoly (oxyethylene)]phenyl]propane(BPE-30). The immobilized microorganism catalyzed the transformation of dehydroepiandrosterone (DHEA) to 16 ahydroxyDHEA. The reaction was followed with the aid of thin-layer chromatography and gas chromatography. The original activity of the free cells was found to be retained about 100% after immobilization. The DHEA-16 α-hydroxylase activity of the entrapped cells in a 5 to 10% dimethyl sulfoxide system was approximately 40 to 80% higher than the original activity of the free cells. The entrapped cells showed maximal steroid 16 a-hydroxylase activity at 4.1 × 10-4mol·dm-4 DHEA. However, at higher concentrations a weak but signi ficant substrate inhibition appeared. The stability of the steroid 16 a-hydroxylase activity of the cells over repeated reactions was greatly improved by immobilization. These results strongly indicate the usefulness of the BPE-30 hydrophilic resin in iffniobilization of S. roseochromo genes having steroid 16 cr-hydroxylation activity.
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