Abstract
A new colorimetric procedure is described for the determination of hydrogen peroxide.This method is based on oxidation reaction between N, N-diethyl-p-phenylenediamine and xanthurenic acid in the presence of peroxidase to form a quinonoid dye.The green chromophore, which is formed in the condition of pH 3.0-4.4, has an absorption peak at 750nm without serum proteins. This system can be utilized for the determination of serum oxalate using oxalate oxidase with the pre-treatment of deproteinization.The determination of serum oxalate can be completed within 15min, and results are linearly related to oxalate concentrations up to 500μM. This procedure is rapid, simple, and precise.
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