국화꽃 물 추출물의 항염 효과

Abstract

Background: The anti-inflammatory effect of Chrysanthmi Flos (CF), primarily produced in the wild and recently being cultivated in Korea was investigated for using it as an anti-inflammatory material.BRMethods and Results: For measuring anti-inflammatory activity, CF was freeze-dried (CFEFD) after water extraction. The production of nitric oxide (NO), prostaglandin E₂ (PGE₂) and proinflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6] via ELISA, and expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), c-Jun Nterminal kinase (JNK)1/2, extracellular-signal-regulated kinase (ERK)1/2, p38, phosphorylated inhibitor of the transcriptional factor NF-κB (IκBα), p65 (a subunit of nuclear factor-κB) protein were determined using Western blot. The production of NO, PGE₂, TNF-α, IL-1β, and IL-6 reduced in a concentration-dependent manner by CFEFD treatment (100 ㎍/㎖ - 400 ㎍/㎖) than that by lipopolysaccharide treatment. iNOS and COX-2 protein expressions were inhibited at a concentration of 100 ㎍/㎖ - 400 ㎍/㎖ and 100 ㎍/㎖ - 200 ㎍/㎖, respectively, by the CFEFD treatment. JNK1/2, ERK1/2, p38 and phosphorylated IκBα protein expressions were inhibited in a concentration-dependent manner by the CFEFD treatment. The expression of p65 protein increased in the cytoplasm and was inhibited in the cell nucleus in a concentration-dependent manner.BRConclusions: Water extract of CF exhibited high anti-inflammatory activity, thus having high value as an anti-inflammatory material.