Abstract

This study analyzed the antibacterial activity of drying methods, including sun drying, hot air drying, and freeze drying of Helianthus tuberosus tea, against acne-causing microorganisms, Candida albicans, Propionibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis. The antibacterial activity of the microorganisms related to acne was searched for extracts of H. tuberosus tea using the paper disk method to measure the antibacterial activity. Among the H. tuberosus tea extracts, the hot air-dried product B and freeze-dried products A and B exhibited antibacterial activity against acne-causing bacteria. Under the condition of culturing acne-causing bacteria together with H. tuberosus tea extract in a liquid medium for 24 hours, strong inhibitory effects were observed in hot air-dried product B and freeze-dried products A and B. The minimum inhibitory concentration (MIC) of H. tuberosus tea against acne-causing bacteria ranged from 0.5 to 1.0 mg eq. for the hot air-dried extract and 0.1 to 0.5 mg eq. for the freeze-dried extract. This finding showed that the freeze-dried product had a lower MIC, indicating higher antibacterial activity. The total polyphenol and flavonoid contents in the six types of H. tuberosus tea were high in the order of sun drying, hot air drying, and freeze drying, indicating apparent differences in the active ingredients depending on the manufacturing method. Considering these results, H. tuberosus tea is believed to have an inhibitory effect against acne-causing bacteria and potential antibacterial effects against acne-causing bacteria.

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