Abstract

When plants are exposed to low temperatures that do not freeze, they receive cold damage, which causes physiological damage, and eventually dies. Recently, the temperature in winter shows a tendency to decrease due to extremely low temperatures, and the cold due to a sudden drop in temperature lasts for several days. As the abnormal low temperature phenomenon (cold wave) causing damage occurs frequently in Jeju Island, it is required to select an appropriate temperature for selection of citrus stock varieties that are resistant to cold damage. The purpose of this study is to select citrus cultivars resistant to cold damage, and to investigate whether shoots are formed in vitro and their growth status in vitro for citrus cultivars mainly used as cultivars. About the shoot formation rate in vitro was investigated, For 13 varieties, in vitro growth status, electrical conductivity, and fatty acid content were investigated. As a result, in vitro shoot formation rate in the Volcano lemon variety showed the highest growth rate compared to other varieties with a shoot formation rate of about 80% or more until 12 hr at low temperature exposure time. I could confirm that. On the other hand, it can be confirmed that the mortality rate of P. trifoliata, which is known as a high-temperature cultivar, was low compared to other varieties when exposed for 24 hours. In addition, at -4℃ in the in vivo growth state, the other cultivars did not die except for some cultivars, but the leaves of most citrus fruits were observed to fall and die in the citrus fruits exposed to -7℃. In addition, at -4℃ in the in vivo growth state, the other cultivars did not die except for some cultivars, but the leaves of most citrus fruits were observed to fall and die in the citrus fruits exposed to -7℃. In addition, in an in vivo experiment, it was confirmed that both leaves and stems receive rapid cold damage when exposed to -5℃ at low temperatures. In addition, the content of saturated fatty acid was increased in general citrus cultivars other than exudate leaves when treated for 12 hours at -4℃. That is, it could be inferred that when the exposure time to low temperature is prolonged, necrosis occurs due to cell membrane destruction due to cold damage. Therefore, it is judged that the proper temperature and time for the selection of citrus stocks that are strong in the cold damage should be treated at -5℃ for 12 hours and irradiated.

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