Abstract
The results of experimental studies on polymorphism of 10 red clover cultivars of Russian selection are presented. Informative primer pairs that can be used for the identification of markers during intervariety differentiation were determined. Based on SSR fingerprints, molecular genetic formulas of the studied cultivars were compiled. Samples of genomic DNA of each cultivar were obtained from pooled aliquots of the plant tissue, each containing 30 seedlings. Eleven pairs of SSR primers developed for structural analysis of the red clover genome were used in genotyping. Unique amplification fragments were found with SSR locus RCS1307 for Trifon and Topaz cultivars, as well as with SSR locus RCS3095 for Trio and Mars cultivars, which can serve as identification DNA markers The results of this study are of practical importance for the molecular genetic certification of breeding achievements, which is necessary to assess the quality and genetic uniformity of seeds and to control hybridization in the breeding process. forage crops, red clover, DNA fingerprinting, SSR loci, cultivar identification This study was funded from the federal budget within the framework of a state assignment (project no. 0442-2019-0001 АААА-А19-119122590053-0).
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