Abstract
The ocean provide great benefits for microalgal mass cultures with maintaining stable temperature due to high specific heat, mixing by wave energy, and providing large area for large-scale microalgae cultures. In this study, we cultivated a marine green microalga, Tetraselmis sp. KCTC12432BP, using marine photobioreactors on the ocean for investigating the effect of NaHCO₃ concentration on the biomass productivities and evaluating the potential of ocean microalgae culture. The culture medium consist of three fold concentrated f/2-Si with 4 g/L of NaHCO3, which is dissolved in natural seawater. After 11 days of cultivation, the cultures reached stationary phase at biomass concentration of 1.6 g/L. At that time, NaHCO₃ concentration of 0, 2, and 4 g/L were fed to the cultures. The daily productivities of 0.11, 0.19, 0.30 g/L/day were attained with feeding rate of 0, 2, and 4 g/L NaHCO₃, respectively. Biomass productivity of Tetraselmis sp. KCTC12432BP was a function of the NaHCO₃ feeding rate as expected. This research shows that the microalgae can grow with NaHCO₃ as carbon source in marine photobioreactors on the ocean while exploiting various benefits of ocean cultivation.
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