Стрессорное воздействие биоцидных метаболитов актинобактерии Streptomyces geldanamycininus Z374 на цианобактерии Microcystis aeruginosa

Abstract

Over the past decades, as a result of anthropogenic pollution and global warming, the frequency, intensity and geographical distribution of the mass development of microalgae and cyanobacteria in water bodies have significantly increased. The Microcystis species are responsible for most cases of the cyanobacterial blooms in freshwater bodies worldwide. Microcystis aeruginosa is the most commonly observed of them. In this work, we assessed the stress effect of isolated from actinobacteria Streptomyces geldanamycininus cyanocidal crude biocide Z374 (CB Z374) on the cyanobacteria M. aeruginosa. The results showed that CB Z374 significantly inhibited M. aeruginosa biomass. The CB Z374 half maximal effective concentration (EC50) for M. aeruginosa after 96 hours of treatment was 5 mg/L. The growth of M. aeruginosa with CB Z374 is characterized by an increase in the duration of the lag-phase, a decrease in the specific growth rate and biomass yield compared to the control. CB Z374 caused oxidative stress in cyanobacteria cells, determined by the elevated levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA) up to 1.6 times, as well as an increased permeability of M. aeruginosa cell membranes by 1.5 times. The levels of superoxide dismutase (SOD) and catalase (CAT) activities and reduced glutathione (GSH) content raised in response to oxidative stress. M. aeruginosa growth inhibition was accompanied by a photosynthesis modification: the photosynthetic pigments phycobiliproteins and carotenoids content increases by 2.3 and 1.6 times respectively and the chlorophyll a content decreases by 1.2 times. CB Z374 inhibited M. aeruginosa photosynthesis, which was expressed in a decline in the maximum quantum efficiency of photosystem II – Fv/Fm by 10% and a reduction in the electron transfer rate (ETR) by 18%. We assume that oxidative stress and, as a result, cell membranes damage and photosynthesis inhibition are the main mechanisms of the cyanocidal action of CB Z374 on M. aeruginosa.