Abstract
Due to maintenance in the leaves of diterpene glycosides of stevia practically used in the world as a substitute of sugar. However much the task of organization of the complex processing of digister costs before processing enterprises. Stevia is an insufficiently known culture that reduces its profitability. Organization of receipt of other matters from the after products of production is possible at more deep research of stevia. It is known that the leaves of stevia contain flavonoids also, determining the amount of which practically is not conducted. In spite absence of control of their maintenance and extraction from the leaves of stevia the concentrates got from them successfully used in the production of cosmetic wares such as hand creams and feet, washed natural and other. The results of scientific researches are in-process presented on development of method of determination of flavonol in leaves the stevia method of spectrophotometric. It is set that the optimum concentration of reagent which is used for besieging of ballast matters of extract got from the dried leaves of stevia is 5% solution of chloride of aluminum in an ethanol. The use of such reagent allows maximally to extract ballast matters from an extract at speed of filtration 1,83 m/s on a vacuum-pump. The optimum entered amount is set: 4 ml of solution of chloride of aluminum in an ethanol on 10 ml of filtrate. Spectrophotometric is got curves of standard solution routine and the probed filtrate. On the basis of maximums of spectrophotometric of curves the general amount of flavonoids is expected in the leaves of stevia, maintenance of which makes 6,39% flavоnol in a count on rutin. Thus, leaves of stevia are the rich source of flavonol that can in future promote profitability of stevia by its deep processing.
Highlights
В работе представлены результаты научных исследований по разработке методики определения флавоноидов в листьях стевии методом спектрофотометрии
Подготовку листьев стевии для анализа проводили следующим образом: в колбу вместимостью 50 мл с притертой пробкой поместили навеску 3 г (±0,1 г) измельченных листьев стевии и добавили нагретую до 35–40 °С дистиллированную воду в соотношении листья – вода как 1 : 10 [10]
The results of scientific researches are in-process presented on development of method of determination of flavonol in leaves the stevia method of spectrophotometric
Summary
В исследовании использовались сушеные листья стевии (Stevia rebaudiana Bertonі), выращенной на опытном участке агрофирмы «Веселиновка» (Киевская область, Украина). Подготовку листьев стевии для анализа проводили следующим образом: в колбу вместимостью 50 мл с притертой пробкой поместили навеску 3 г (±0,1 г) измельченных листьев стевии и добавили нагретую до 35–40 °С дистиллированную воду в соотношении листья – вода как 1 : 10 [10]. Экстрагирование проводили в термостате при температуре 60 °С на протяжении 30 мин. После чего добавили 10 мл этилового спирта и продолжили экстракцию еще 30 мин. Приготовление раствора хлорида алюминия в этаноле делали по методике В.Л. Измерения проводили на спектрофотометре СФ-46 при длине волн 380–420 нм в кювете толщиной 1 см
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