Abstract
ABSTRACT This study investigates the in-situ implementation of bio-regenerated iron mineral catalyst to remove explosive compoundsin ground water at a military shooting range in operation. A bio-regenerated iron mineral catalyst was synthesized usinglepidocrocite (iron-bearing soil mineral), iron-reducing bacteria Shewanella putrefaciens CN32, and electron mediator(riboflavin) in the culture medium. This catalyst was then injected periodically in the ground to build a redox active zoneacting like permeable reactive barrier through injection wells constructed at a live fire military shooting range. Groundwater and core soils were sampled periodically for analysis of explosive compounds, mainly RDX and its metabolites,along with toxicity analysis and REDOX potential measurement. Results suggested that a redox active zone was formed inthe subsurface in which contaminated ground water flows through. Concentration of RDX as well as toxicity (% inhibition)of ground water decreased in the downstream compared to those in the upstream while concentration of RDX reductionproducts increased in the downstream. Key words : Electron transfer mediator, Iron reducing bacteria, Lepidocrocite, RDX, TNX
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