Abstract
The aim of this study was to isolate and investigate enzymes involved in the biodegradation of perfluorocarboxylic acids (PFCAs) in Pseudomonas plecoglossicida strain 2,4-D and Pseudomonas mosselii strain 5(3). In this research, enzymes from these strains were concentrated from the culture medium by ultrafiltration and ammonium sulfate precipitation, followed by purification through size-exclusion chromatography. The enzyme activity after ultrafiltration was higher Enzymes from strain 2,4-D released 2 ppm of fluoride ions after 24 hours, increasing to 40,7 ppm after 48 hours. For strain 5(3), fluoride release reached 3 ppm at 24 hours and 46 ppm at 48 hours, confirming the activity of the isolated dehalogenases and their ability to degrade PFCAs. Size-exclusion chromatography revealed that the molecular weight of enzymes from strain 5(3) is approximately 80 kDa, while for strain 2,4-D, it is 76 kDa. The study of these enzymes holds both applied significance for environmental remediation technology development and fundamental importance. Understanding the molecular structure and mechanisms of dehalogenase activity not only provides insights into the cleavage of robust C-F bonds but also sheds light on enzyme evolution as a whole.
Published Version
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