Abstract
The nucleotide sequence coding N-terminal module of Bos taurus tyrosyl-tRNA synthetase (mini TyrRS) was cloned into the bacterial expression vector pET23d(+). Bacterial expression of the recombinant protein mini TyrRS was performed in E. coli BL21 (DE3)pLysE cells with the use of the constructed vector pET-23d(+)39YRS for subsequent physical and chemical protein studies. The catalytic activity of the recombinant mini TyrRS has been studied in the aminoacylation reaction of homologous tRNATyr.
Highlights
For the right side of the thoracic spines: Th6 – 0,85 0,73 and -0,79 7,49, Th7 – -1,00 0,79 and -1,37 0,69, Th8 – -0,96 0,73 and -0,99 0,68, Th9 – -0,12 0,64 and -0,38 0,83, Th10 – -0,92 1,14 and -1,03 1,00, Th11 – -1,69 1,05 and -1,86 1,06, Th12- -1,65 1,15 and -1,96 1,12 respectively
another connected with the autonomic nervous system
real to evaluate the functional state of the person by temperature characteristics
Summary
For the right side of the thoracic spines: Th6 – 0,85 0,73 (control) and -0,79 7,49 (experiment), Th7 – -1,00 0,79 and -1,37 0,69, Th8 – -0,96 0,73 and -0,99 0,68, Th9 – -0,12 0,64 and -0,38 0,83, Th10 – -0,92 1,14 and -1,03 1,00, Th11 – -1,69 1,05 and -1,86 1,06, Th12- -1,65 1,15 and -1,96 1,12 respectively. БАКТЕРІАЛЬНА ЕКСПРЕСІЯ РЕКОМБІНАНТНОГО ФУНКЦІОНАЛЬНО АКТИВНОГО N-КІНЦЕВОГО МОДУЛЯ ТИРОЗИЛ-ТРНК СИНТЕТАЗИ B. Проведено клонування в експресуючий вектор рЕТ23d(+) кДНК, кодуючої N-кінцевий каталітичний модуль тирозилтРНК синтетази (міні TyrRS) Bos taurus.
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